The couple, who had displayed the llama-like animals at an August show, left them in a trailer while going shopping.
They were then heading home from Stratford-upon-Avon, Warks, when Travelodge staff alerted them.
The sheepish owners collected the pets eight hours after leaving.
Travelodge spokeswoman Shakila Ahmed said: “They had been into town and had some lunch and were some 30 miles down the motorway when they realised what they’d done.
“Staff had to keep a close eye on the alpacas to make sure they were OK throughout the afternoon.”
Other lost property at the chain’s 571 hotels last year included an Aston Martin car, a 65-year-old bonsai tree and a gingerbread village.
A cat show enthusiast staying in Stirling realised she had forgotten her Persian chinchilla cat, Angel, 50 miles into her car journey.
And dedicated staff at London City Travelodge rushed to Heathrow Airport to reunite a customer with his Tiffany engagement ring he had placed in his pillowcase.
Shakila said: “With nearly 19 million customers annually, we do get a range of interesting items left behind. In the rush valuable things are forgotten.
The most common items customers forget include laptops, tablets, e-readers and phones – and their chargers.
(Story source: The Sun)